Guidelines for sample submission:
Please contact Microarray Facility when planning GeneChip experiment to ensure the availability of the number of chips to be used. It usually takes one to two weeks to order and receive the chips.
There are two options for sending samples:
Schedule an appointment to bring samples to the facility or Send samples by Campus Mail. In this case samples should be packed properly for shipping and the facility should be notified in advance.
Completion of Sample submission form is required at the time of sample submission. Sample names must be clearly labeled on top of the tube and must match names on the request form or they will be returned. Sample names cannot be longer than 12 characters in length. All RNA samples must be DNase treated prior to submission to ensure all contaminating genomic DNA has been removed. We recommend that you resuspend or elute your RNA in DEPC water.
If you are submitting samples for nucleic acid quantification via the Nanodrop you must submit an aliquot of the elution buffer with your samples. For Nanodrop quantification please submit 3 ul of your sample.
The quality of total RNA has to be check via 2100Bioanalyzer (Agilent Technology). 2 ul of sample should be submitted for Bioanalazer analysis.
Investigators are responsible for the cost of RNA concentration and quality assessment performed in Genomics Core Facility, unless they can provide Nanodrop and Bioanalyzer files / printouts at the time of sample submission.
Samples that do not pass the QC metrics will not be processed on a microarray.