GeneChip Hybridization

The University of Tulsa
Microarray Core Facility
Department of Biological Science
800 South Tucker Drive Tulsa, OK 74104

Services

	Total RNA extraction
	mRNA quality control utilizing the BioAnalyzer
	Quantified using the NanoDrop ND-1000
	Clean-up of total RNA using Qiagen RNeasy
	Sample target labeling
	GeneChip Hybridization
	Array Scanned and quantified using GCOS

Turn around time depends on the number of chips requested by a customer and usage. Please contact us for more information.

Submitting RNA samples for the GeneChip® Hybridization

RNA should be stored at -80°C once it is extracted and shipped in dry ice by overnight mail or hand delivered in dry ice. RNA with ethanol and salt (RNA later) can also be shipped in dry ice by overnight mail or hand delivered in dry ice.

Samples for One-Cycle Labeling should be shipped at a standard concentration of 1µg/µL and a minimum mass of 40µg. Two-Cycle Labeling protocols call for a standard concentration of 25ng/µL and a minimum mass of 100ng. Re-concentration could effect the integrity of the RNA. The TU Microarry Core Laboratory is not responsible for costs associated with insufficient concentration and poor quality RNA.

Samples that do not pass the QC metrics will not be processed on a microarray.

Mailing and hand delivery address: Jacob P. Crowley; Department of Biological Science (Oliphant Hall Room N203); The University of Tulsa; 800 South Tucker Drive; Tulsa, OK 74104-3189; jacob-crowley@utulsa.edu; Tel 918-631-2760; Fax 918-631-2762

Services

Submitting RNA samples for the GeneChip® Hybridization

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